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Guerfel et al

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The absorbance at 490 nm was measured and the concentration of FITC-labeled dextran was calculated from the standard curve for the upper and lower chambers in each group before calculating the penetration rate

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In the stimulated group, platelet aggregates rapidly formed on the endothelial surface, and continued to accumulate over time, while leukocytes adhered to the vessel walls and began migrating through the endothelium into the collagen matrix after 1 h of blood perfusion